In eukaryotic lineages with monocentric chromosomes, centromeres and centromeric histone H3 (CENH3) undergo a recurrent evolutionary conflict, whereby selfish centromeres subvert female meiosis, and CENH3 evolves adaptively to counterbalance the deleterious consequences of centromere drive. Positively selected CENH3 can therefore provide an indication as to whether this evolutionary arms race has occurred in a given lineage.
Zedek and Bureš analysed selective pressures acting on CENH3 in Luzula (Juncaceae), a genus with holokinetic chromosomes. They found an absence of positive selection, concluding that holokinetic chromosomes could have evolved as a defence against centromere drive.
Zedek, F., & Bureš, P. (2016). Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive. Annals of Botany, 118(7), 1347–1352. https://doi.org/10.1093/aob/mcw186