Does removal of secondary metabolites improve Zingiberaceous nuclei quality?

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The family Zingiberaceae is well known for its range of secondary constituents and bioactive metabolites, such as flavonoids, phenolic acids and essential oils known to increase nuclei stickiness in cell free lysate.

Photomicrographs obtained from bio-SEM analyses of A. zerumbet shoot nuclear suspensions using different isolation buffers.
Photomicrographs obtained from bio-SEM analyses of A. zerumbet shoot nuclear suspensions using different isolation buffers. (A) Arumuganathan and Earle buffer, (B) Galbraith’s buffer, (C) General purpose buffer, (D) LB01 buffer, (E) Marie’s nuclear isolation buffer, (F) MB01 buffer, (G) Otto buffers, (H) Tris-MgCl2 buffer, (I) Tris-MgCl2 buffer with 1 % PVP. Image: Sadhu et al. 2016.

As single nuclei of correct shape and size are the prerequisites for accurate genome size estimation by flow cytometry, Sadhu et al. present a new buffer for nuclei isolation, MB01, that provides good quality cytograms and stain–nuclei interactions. Comparison with widely used isolation buffers proved MB01’s superiority. MB01 also helped to estimate successfully genome sizes of 14 unmeasured Zingiberaceous species.

Reference

Abhishek Sadhu, Sreetama Bhadra, Maumita Bandyopadhyay, 2016, 'Novel nuclei isolation buffer for flow cytometric genome size estimation of Zingiberaceae: a comparison with common isolation buffers', Annals of Botany, vol. 118, no. 6, pp. 1057-1070 http://dx.doi.org/10.1093/aob/mcw173


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