Annals of Botany

Mapping developmental zones at the root apex using the multiple structural change algorithm

In the root apex, cell length is a function of its position within a cell file. Applying a mathematical approach using a multiple structural change (MSC) algorithm, Pacheco-Escobedo et al. developed a spatial model to identify that the growing part of Arabidopsis root is composed of three discrete developmental zones: the proliferation domain (PD), the transition domain (TD), and the elongation zone (EZ).

Determination of the PD/TD boundary in the CCS52A1:GUS line using the MSC approach
Determination of the PD/TD boundary in the CCS52A1:GUS line using the MSC approach; GUS expression marks the beginning of endoreduplication. (A–J) CCS52A1:GUS expression in wt roots (7 DAS) occurs in the TD and EZ. Yellow rectangles indicate the 95 % CI of the position of the last PD cell estimated by the MSC approach. Scale bar = 50 μm.

The PD and the TD comprise the root apical meristem (RAM). The MSC approach enables unambiguous, rapid, and automated determination of the RAM/ EZ and the PD/TD boundaries. This publicly available tool facilitates root phenotyping of different genetic backgrounds and experimental treatments.

Root Biology Issue This paper is part of the Root Biology Special Issue.

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