In the root apex, cell length is a function of its position within a cell file. Applying a mathematical approach using a multiple structural change (MSC) algorithm, Pacheco-Escobedo et al. developed a spatial model to identify that the growing part of Arabidopsis root is composed of three discrete developmental zones: the proliferation domain (PD), the transition domain (TD), and the elongation zone (EZ).
The PD and the TD comprise the root apical meristem (RAM). The MSC approach enables unambiguous, rapid, and automated determination of the RAM/ EZ and the PD/TD boundaries. This publicly available tool facilitates root phenotyping of different genetic backgrounds and experimental treatments.
This paper is part of the Root Biology Special Issue.
Mario A. Pacheco-Escobedo, Victor B. Ivanov, Iván Ransom-Rodríguez, Germán Arriaga-Mejía, Hibels Ávila, Ilya A. Baklanov, Arturo Pimentel, Gabriel Corkidi, Peter Doerner, Joseph G. Dubrovsky, Elena R. Álvarez-Buylla, Adriana Garay-Arroyo, 2016, 'Longitudinal zonation pattern in Arabidopsis root tip defined by a multiple structural change algorithm', Annals of Botany, vol. 118, no. 4, pp. 763-776 http://dx.doi.org/10.1093/aob/mcw101