Using an immunochemical approach to reveal the link between changes in abscisic acid (ABA) levels, aquaporin function and changes in root cell hydraulic conductivity (LpCell), Sharipova et al. compared the abundance of AQPs and ABA by means of immunolocalization in the tissue of barley (Hordeum vulgare) roots, and related it to LpCell.
Shortly after (< 2 hours) ABA application to the roots of ABA-deficient barley, increased tissue ABA concentrations and AQP abundance (especially the plasma-membrane localised isoforms HvPIP2;1 and HvPIP2;2) were spatially correlated in root epidermal cells and the cortical cell layer located beneath, in conjunction with increased LpCell of the cortical cells.
This paper is part of the Root Biology Special Issue.
Guzel Sharipova, Dmitriy Veselov, Guzel Kudoyarova, Wieland Fricke, Ian C. Dodd, Maki Katsuhara, Takuya Furuichi, Igor Ivanov, Stanislav Veselov, 2016, 'Exogenous application of abscisic acid (ABA) increases root and cell hydraulic conductivity and abundance of some aquaporin isoforms in the ABA-deficient barley mutant Az34', Annals of Botany, vol. 118, no. 4, pp. 777-785 http://dx.doi.org/10.1093/aob/mcw117