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Home Journals Annals of Botany Method for transient gene expression in monocots

Method for transient gene expression in monocots

Method for transient gene expression in monocots
Method for transient gene expression in monocots

There are a number of well-developed Agrobacterium-mediated transformation methods for dicotyledonous plants, but few for monocotyledonous plants. Fursova et al. develop a new approach for transient gene expression in the grass, Brachypodium distachyon, using co-cultivation of mature seeds with bacterial cells, and including leaf extract of Nicotiana tabacum and acetosyringone. Expressed proteins are directed to a plant apoplast, where they maintain hydrolytic activity and alter cell wall composition and biomass digestibility. This newly developed approach provides a quick and efficient technique for expressing genes of interest in Brachypodium.

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