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Method for transient gene expression in monocots

Method for transient gene expression in monocots
Method for transient gene expression in monocots

There are a number of well-developed Agrobacterium-mediated transformation methods for dicotyledonous plants, but few for monocotyledonous plants. Fursova et al. develop a new approach for transient gene expression in the grass, Brachypodium distachyon, using co-cultivation of mature seeds with bacterial cells, and including leaf extract of Nicotiana tabacum and acetosyringone. Expressed proteins are directed to a plant apoplast, where they maintain hydrolytic activity and alter cell wall composition and biomass digestibility. This newly developed approach provides a quick and efficient technique for expressing genes of interest in Brachypodium.

Written by Annals of Botany Office

The Annals of Botany Office is based at the University of Oxford.

Image: from Shokoku meisho hyakkei by Hiroshige II (Chinpei Suzuki), 1860.

Lichens, thale cress, tobacco, the great survivors…

Image: Wikimedia Commons.

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