I was looking at the downloads of papers from Annals of Botany over the last year. In the top ten of most downloaded papers was one from 1997 (certainly when I relied on photocopiers rather than PDFs). It was on the Brazilian cerrado vegetation and threats to its biodiversity. I expect this was a paper long before its time, but now the threats to the diversity of this ecosystem are becoming recognized widely. Hopefully we are in time to conserve the 160,000 species the paper estimates are present in this diversity hotspot.
J RATTER, 1997, 'The Brazilian Cerrado Vegetation and Threats to its Biodiversity', Annals of Botany, vol. 80, no. 3, pp. 223-230 http://dx.doi.org/10.1006/anbo.1997.0469